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Optimax Systems Inc multigene optimax thermal cycler
Multigene Optimax Thermal Cycler, supplied by Optimax Systems Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multigene+optimax+thermal+cycler/cycler+multigene+optimax+tc9610+thermal/10__1017_slash_s0954102026100649-70-17-18
Average 86 stars, based on 1 article reviews
multigene optimax thermal cycler - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Urokinase-type plasminogen activator-mediated crosstalk between N-cadherin and β-catenin promotes wound healing
Article Snippet: PCR RNA was extracted with a Rneasy mini kit (Qiagen, Germantown, MD) and quantified with a Nanodrop system from Wt cerebral cortical astrocytes treated for 24 h with 5 nM of uPA, 1 μM of XAV-939, or a combination of uPA and XAV-939. cDNA was prepared from 500 ng of RNA using the iScript cDNA Synthesis kit. .. Then, a PCR product to detect Myc , Plaur or Actb was generated with a MultiGene OptiMax Thermal Cycler [72°C×2 min; 30 cycles of 98°C×10 s, 60°C×30 s, 72°C×20 s, and 72°C×10 min (Labnet International, Edison, NJ)], an EmeraldAMp Max HS PCR master mix, and the primers described above. .. The amplified PCR product was detected with a 2% agarose gel and measured with the LI-COR Odyssey Fc reader and IMAGE STUDIO ver 5.2 (Li-Cor Lincoln, NE).

Article Title: Fine‐scale hierarchical genetic structure and kinship analysis of the ascidian Pyura chilensis in the southeastern Pacific
Article Snippet: .. Ten polymorphic microsatellite regions developed previously for this species were amplified by PCR on a MultiGene OptiMax Thermal Cycler and run on an ABI 3500 fragment analyzer (Applied Biosystems) available at the AUSTRAL‐omics core facility of the Faculty of Science at the Universidad Austral de Chile ( www.australomics.cl ). ..

Article Title: The unique origin of orange carrot cultivars in China
Article Snippet: Cultivated carrots can be divided into eastern and western types.. Much evidence supports the idea that eastern carrots originated in Central Asia, while varying opinions exist on the origin of western carrots, especially orange varieties, and the origin of Chinese orange carrots remains unclear.. In this study, we used 119 carrot accessions to investigate the relationship between Chinese carrots and western orange varieties (Western orange) using morphology and simple sequence repeat (SSR) markers.

Article Title: Swarming in the storm: Collembola aggregations on sub-Antarctic Marion Island
Article Snippet: .. The individual springtails were then homogenized using a pestle, and each PCR tube was placed into a MultiGene OptiMax thermal cycler with the following conditions: initial denaturation at 96○C for 5 min, 40 cycles of 30 s at 96○C for denaturation, 30 s at 48○C for annealing and 50 s at 72○C for extension, followed by a final extension step at 72○C for 10 min. All samples underwent postPCR clean-up using the Macherey-Nagel NucleoSpin® Gel and PCRClean-upKit following themanufacturer’s recommendations. .. Successful amplifications were confirmed using 2% agarose gel electrophoresis with 0.5X TBE buffer and the GelRed Nucleic Acid Gel Stain (Biotium).

Generated:

Article Title: Urokinase-type plasminogen activator-mediated crosstalk between N-cadherin and β-catenin promotes wound healing
Article Snippet: PCR RNA was extracted with a Rneasy mini kit (Qiagen, Germantown, MD) and quantified with a Nanodrop system from Wt cerebral cortical astrocytes treated for 24 h with 5 nM of uPA, 1 μM of XAV-939, or a combination of uPA and XAV-939. cDNA was prepared from 500 ng of RNA using the iScript cDNA Synthesis kit. .. Then, a PCR product to detect Myc , Plaur or Actb was generated with a MultiGene OptiMax Thermal Cycler [72°C×2 min; 30 cycles of 98°C×10 s, 60°C×30 s, 72°C×20 s, and 72°C×10 min (Labnet International, Edison, NJ)], an EmeraldAMp Max HS PCR master mix, and the primers described above. .. The amplified PCR product was detected with a 2% agarose gel and measured with the LI-COR Odyssey Fc reader and IMAGE STUDIO ver 5.2 (Li-Cor Lincoln, NE).

Amplification:

Article Title: Limitations of DNA barcoding in determining the origin of smuggled seahorses and pipefishes
Article Snippet: .. Amplification was conducted on a MultiGene OptiMax thermal cycler using the following conditions: a denaturing step at 96 C for 5 min, 40 cycles of 30 s at 96 C for denaturation, 2 min at 49 C for annealing and 50 s at 72 C for extension, followed by a final extension 72 C for 10 min. .. Successful amplification was confirmed using 2% agarose gel electrophoresis with 0.5X TBE buffer, and the bands were visualized using GelRed Nucleic Acid Gel Stain (Biotium).

Article Title: Fine‐scale hierarchical genetic structure and kinship analysis of the ascidian Pyura chilensis in the southeastern Pacific
Article Snippet: .. Ten polymorphic microsatellite regions developed previously for this species were amplified by PCR on a MultiGene OptiMax Thermal Cycler and run on an ABI 3500 fragment analyzer (Applied Biosystems) available at the AUSTRAL‐omics core facility of the Faculty of Science at the Universidad Austral de Chile ( www.australomics.cl ). ..



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